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您現(xiàn)在的位置:首頁  >  技術(shù)文章  >  mVSMC-pds151細(xì)胞 現(xiàn)貨附STR鑒定

mVSMC-pds151細(xì)胞 現(xiàn)貨附STR鑒定

發(fā)布時(shí)間:2017-05-24瀏覽:956次

mVSMC-pds151細(xì)胞| mVSMC-pds151細(xì)胞株 現(xiàn)貨附STR鑒定

 

產(chǎn)品名稱:MC3T3-E1細(xì)胞

種屬: 小鼠顱骨前骨細(xì)胞

生長特性:貼壁

形態(tài): 成纖維細(xì)胞形態(tài)

培養(yǎng)基: 90% MEM+10%FBS

生長條件: 95%空氣+5%二氧化碳 37攝氏度

凍存條件: 90%FBS+10%DMSO

 

上?;鄯f生物是一家專門從事細(xì)胞培養(yǎng)相關(guān)試劑公司,擁有完善細(xì)胞庫管理體系,公司4000多株細(xì)胞逐一做STR鑒定,為每個(gè)科研用戶負(fù)責(zé),提供無交叉污染,真實(shí),來源可靠的細(xì)胞株!

 

mVSMC-pds151細(xì)胞| mVSMC-pds151細(xì)胞株 現(xiàn)貨附STR鑒定

常見抗生素的配方:

氨芐青霉素

 

氨芐青霉素(ampicillin)(100mg/ml)縮寫:Amp

溶解1g氨芐青霉素鈉鹽于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以25ug/ml~50ug/ml的終濃度添加于生長培養(yǎng)基。

 

羧芐青霉素

 

羧芐青霉素(carbenicillin)(50mg/ml)縮寫:Cab

溶解0.5g羧芐青霉素二鈉鹽于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以25ug/ml~50ug/ml的終濃度添加于生長培養(yǎng)基。

 

甲氧西林

 

甲氧西林(methicillin)(100mg/ml)

溶解1g甲氧西林鈉于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以37.5ug/ml終濃度與100ug/ml氨芐青霉素一起添加于生長培養(yǎng)基。

 

卡那霉素

 

卡那霉素(kanamycin)(10mg/ml)縮寫:Kan

溶解100mg卡那霉素于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以10ug/ml~50ug/ml的終濃度添加于生長培養(yǎng)基。

氯霉素

 

氯霉素(chloramphenicol)(25mg/ml)縮寫:Cm

溶解250mg氯霉素足量的無水乙醇中,zui后定容至10ml。分裝成小份于-20℃貯存。常以12.5ug/ml~25ug/ml的終濃度添加于生長培養(yǎng)基。

生物實(shí)驗(yàn)高清視頻教程

 

鏈霉素

 

鏈霉素(streptomycin)(50mg/ml)縮寫:Sm

溶解0.5g鏈霉素硫酸鹽于足量的無水乙醇中,zui后定容至10ml。分裝成小份于-20℃貯存。常以10ug/ml~50ug/ml的終濃度添加于生長培養(yǎng)基。

 

萘啶酮酸

 

萘啶酮酸(nalidixic acid)(5mg/ml)

溶解50mg萘啶酮酸鈉鹽于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以15ug/ml的終濃度添加于生長培養(yǎng)基。

 

四環(huán)素

 

四環(huán)素(tetracyyline)(10mg/ml)縮寫:Tet

溶解100mg四環(huán)素鹽酸鹽于足量的水中,或者將無堿的四環(huán)素溶于無水乙醇,定容至10ml。分裝成小份用鋁箔包裹裝液管以免溶液見光,于-20℃貯存。常以10ug/ml~50ug/ml的終濃度添加于生長培養(yǎng)基。(注:鎂離子是四環(huán)素的拮抗劑,四環(huán)素抗性菌的篩選應(yīng)使用不含鎂鹽的培養(yǎng)基,如LB培養(yǎng)基。)

 

紅霉素

 

紅霉素(erythromycin)(20mg/ml)縮寫:Em

容積為95%乙醇或冰醋酸,用錫箔包裹以免溶液見光,于-20℃貯存,稀釋劑為蒸餾水。

(紅霉素和四環(huán)素等在偏酸環(huán)境下抗菌活性是會(huì)下降的,而在偏堿環(huán)境則會(huì)增加,阿米卡星和頭孢類等在偏堿環(huán)境抗菌活性會(huì)下降,在偏酸環(huán)境是會(huì)增加。)

 

mVSMC-pds151細(xì)胞| mVSMC-pds151細(xì)胞株 現(xiàn)貨附STR鑒定

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